Part:BBa_K3237022:Design
HIS-mRFP1-SCI57-PTDwith furin cut sites codon optimized for E.coli
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1745
Illegal XbaI site found at 1733
Illegal XbaI site found at 1775
Illegal SpeI site found at 1769
Illegal PstI site found at 1283
Illegal PstI site found at 1751 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1745
Illegal NheI site found at 1297
Illegal SpeI site found at 1769
Illegal PstI site found at 1283
Illegal PstI site found at 1751
Illegal NotI site found at 1781 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1745
Illegal BamHI site found at 1763 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1745
Illegal XbaI site found at 1733
Illegal XbaI site found at 1775
Illegal SpeI site found at 1769
Illegal PstI site found at 1283
Illegal PstI site found at 1751 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1745
Illegal XbaI site found at 1733
Illegal XbaI site found at 1775
Illegal SpeI site found at 1769
Illegal PstI site found at 1283
Illegal PstI site found at 1751
Illegal AgeI site found at 881
Illegal AgeI site found at 993 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part was codon optimized for E.coli .
Source
he mRFP1 sequence is based off of part BBa_E1010 and the SCI57 sequence was found in the literature [1]. The construct itself was synthesized by TWIST.
References
1. Hua, Q., Nakagawa, S., Jia, W., Huang, K., Philips, N., Hu, S., and Wiess, M. (2008) Design of an active ultrastable single-chain insulin analog: synthesis, structure, and therapuetic implications. The Journal of Biological Chemistry. 21, 1473-14716.
2.Liang, J., and Yang, V. (2005) Insulin-cell penetrating peptide hybrids with improved intestinal absorption efficiency. Communications. 335, 734-738.
3. Hay, C., and Docherty, K. (2003) Enhanced expression of a furin-cleavable proinsulin. Journal of molecular endocrinology. 3, 597-607.